Figure 1. FIGURE 1: Growth rate, budding percentage, and trehalose and glycogen accumulation upon deprivation for iron or zinc.

All experiments were performed with the Σ1278 wild type strain. All samples originate from a preculture that was grown overnight in all trace medium, and subsequently diluted at t = 0 to an OD₆₀₀ of 0.5 in the indicated deprivation media.

(A) Growth inhibition upon exposure to different iron or zinc deprivation conditions, n = 2. Cells were put on fresh deprivation medium each 24 h and OD₆₀₀ was measured at regular intervals. (closed circles) all trace medium, (open circles) iron deprivation with BPS, (closed squares) iron deprivation with ferrozine, (closed triangles) zinc deprivation with EDTA.

(B) Percentage of unbudded cells in response to the indicated deprivation conditions with CSM complete as control condition, n = 2.

(C) Accumulation of trehalose in response to the indicated deprivation conditions, n = 2.

(D) Accumulation of glycogen in response to the indicated deprivation conditions, n = 2.

(B,C,D) The shaded bars indicate non-starved cells and 1, 2, 3 or 4 days of metal ion starvation.