Table 3.
List of primers used for site-directed mutagenesis of Zrt1.
| Name | Mutation | Sequence |
| Zrt1-D105N Fw | ZRT1D105N | CACTTAATGAACCCTGCTTAT |
| Zrt1-D105N Rv | ZRT1D105N | ATAAGCAGGGTTCATTAAGTG |
| Zrt1-D144N Fw | ZRT1D144N | TTCCTTACTAATCTATTCAGT |
| Zrt1-D144N Rv | ZRT1D144N | ACTGAATAGATTAGTAAGGAA |
| Zrt1-E226Q Fw | ZRT1E226Q | TTAATTTTACAATTCGGTGTG |
| Zrt1-E226Q Rv | ZRT1E226Q | CACACCGAATTGTAAAATTAA |
| Zrt1-E261Q Fw | ZRT1E261Q | CAATCATTTCAAGGTTTAGGT |
| Zrt1-E261Q Rv | ZRT1E261Q | ACCTAAACCTTGAAATGATTG |
| Zrt1-E273Q Fw | ZRT1E273Q | TCAGCCATTCAATTCCCTAGA |
| Zrt1-E273Q Rv | ZRT1E273Q | TCTAGGGAATTGAATGGCTGA |
| Zrt1-D322N Fw | ZRT1D322N | GGTGTTTTGAATGCCATTTCT |
| Zrt1-D322N Rv | ZRT1D322N | AGAAATGGCATTCAAAACACC |
| Zrt1-E336Q Fw | ZRT1E336Q | GGTTTGGTTCAACTACTAGCA |
| Zrt1-E336Q Rv | ZRT1E336Q | TGCTAGTAGTTGAACCAAACC |
| Zrt1-E354Q Fw | ZRT1E354Q | GATCTAAGACAATTGTCCTTC |
| Zrt1-E354Q Rv | ZRT1E354Q | GAAGGACAATTGTCTTAGATC |