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. 2015 Oct 12;2(11):412–428. doi: 10.15698/mic2015.11.237

Table 2.

Combinations of embedding media, fixation methods and staining procedures employed for yeast ultrastructural analyses.

Epon Spurr's LR White Lowicryl HM20 Tokuyasu preparations
Fixation
Glutaraldehyde (X) (X) X X X
Paraformaldehyde ND (X) X X X
Potassium permanganate ND X ND ND ND
HPF/FS X (X) (X) X (X)
Staining
Osmium tetroxide X (X) ND X ND
Uranyl acetate X X X X X
Lead citrate X X X ND (X)
Tannic acid X ND ND (X) ND