Table 1.
Apoptotic cells and fold changes in fluorescence intensity of MICA-S and MICA-NS C. parapsilosis strains treated with micafungin.
‘-’ Not detected (0% of cells showed particular apoptotic marker).
PI, propidium iodide; ROS, reactive oxygen species; ΔΨm, mitochondrial membrane potential; NAC, N-acetyl cysteine.
| Strains | Apoptotic Cells | |||||
| Protoplast % | Fold change | |||||
| C. parapsilosis | Annexin V | PI | ROS | ΔΨm | ||
| -NAC | +NAC | -NAC | +NAC | |||
| Isolate-1 (MICA-S) | ||||||
| Control | 5.0 ± 0.01 | - | - | - | - | - |
| 0.25 mg/L | 24.0 ± 2.0 | - | 1.8 ± 0.01 | 1.5 ± 1.00 | 2.8 ± 0.1 | 1.9 ± 0.01 |
| 0.5 mg/L | 52.0 ± 3.0 | 2.0 ± 0.01 | 2.4 ± 0.2 | 1.68 ± 0.20 | 3.8 ± 0.4 | 2.48 ± 0.01 |
| 1.0 mg/L | 19.0 ± 1.0 | 9.0 ± 1.0 | 1.9 ± 0.01 | 1.61 ± 0.21 | 2.5 ± 0.2 | 1.8 ± 0.01 |
| Isolate-6 (MICA-NS) | ||||||
| Control | 2.0 ± 0.01 | 1.0 ± 0.01 | - | - | - | - |
| 0.5 mg/L | 7.0 ± 1.0 | - | 1.5 ± 0.01 | 1.0 ± 0.02 | 1.9 ± 0.01 | 1.4 ± 0.10 |
| 1.0 mg/L | 30.0 ± 2.0 | - | 1.4 ± 0.01 | 1.3 ± 0.01 | 1.98 ± 0.01 | 0.89 ± 0.01 |
| 2.0 mg/L | 11.0 ± 1.0 | 6.0 ± 0.01 | 1.6 ± 0.01 | 1.1 ± 0.20 | 2.0 ± 0.01 | 1.4 ± 0.16 |