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. 2014 Aug 1;1(8):256–266. doi: 10.15698/mic2014.08.160

Table 2.

G1 parameters of "growth" and cell cycle mutantsa.

aThe strains were examined in at least one experiment in each background, and in each case a technical duplicate was evaluated. The cells were cultured in 1% w/v yeast extract, 2% w/v peptone, 2% w/v Dextrose. All the parameters were calculated as described in Table 1 and in the Materials and Methods section.

ORF Strain Birth size (fL) k (h-1) Critical size (fL) TG1 (h)
NA BY4741 21.9 0.35 41.2 1.81
ACS1 acs1::KanMX 20.2 0.3 39.1 2.17
ADK1 adk1::KanMX 33 0.25 66.6 2.82
BCK2 bck2::KanMX 28.3 0.35 49.9 1.64
CLN3 cln3::KanMX 33.6 0.41 120 3.14
EMI5 emi5::KanMX 18.8 0.29 40 2.62
GLT1 glt1::KanMX 21.4 0.27 40.2 2.32
HXK2 hxk2::KanMX 15.6 0.3 37.4 2.88
IDH2 idh2::KanMX 24.1 0.27 40.1 1.92
LPD1 lpd1::KanMX 21.1 0.33 46.1 2.34
OAR1 oar1::KanMX 21.6 0.3 44.9 2.47
RPL20B rpl20b::KanMX 16.7 0.26 37.1 3.08
RPS0B rps0b::KanMX 15 0.25 44.5 4.37
SCH9 sch9::KanMX 17 0.29 44.2 3.25
SOL3 sol3::KanMX 22.4 0.3 40.8 1.98
SPE1 spe1::KanMX 25.8 0.26 36.9 1.37
TDA1 tda1::KanMX 24.4 0.33 42 1.64
TOR1 tor1::KanMX 17.9 0.35 42.3 2.47
WHI5 whi5::KanMX 17.3 0.19 25.5 2.04
NA Y7092 19.5 0.35 46.9 2.53
ACS1 acs1::NatMX 19.4 0.28 41.1 2.71
ADK1 adk1::NatMX 23.7 0.21 76.3 5.54
BCK2 bck2::NatMX 22.4 0.31 52.3 2.79
CLN3 cln3::NatMX 27.9 0.39 94.1 3.14
EMI5 emi5::NatMX 18.5 0.34 44.5 2.56
GLT1 glt1::NatMX 20.4 0.27 43.3 2.77
HXK2 hxk2::NatMX 14.3 0.2 35.2 4.48
IDH2 idh2::NatMX 19.4 0.29 45.4 2.91
LPD1 lpd1::NatMX 18.6 0.29 46.1 3.16
OAR1 oar1::NatMX 18.2 0.25 41.5 3.31
RPL20B rpl20b::NatMX 14.6 0.23 39.8 4.35
RPS0B rps0b::NatMX 15.5 0.2 42.7 5.08
SCH9 sch9::NatMX 18.9 0.3 42.5 2.73
SOL3 sol3::NatMX 15.5 0.38 42.4 2.65
SPE1 spe1::NatMX 23.7 0.34 42.8 1.73
TDA1 tda1::NatMX 19.7 0.28 39.3 2.5
TOR1 tor1::NatMX 14.3 0.28 40.8 3.81
WHI5 whi5::NatMX 17.3 0.19 25.3 2