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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Ann Rheum Dis. 2015 Oct 9;75(9):1706–1713. doi: 10.1136/annrheumdis-2015-207593

Figure 2. Blocking Netrin-1 using monoclonal antibody injections decreases bone pitting, inflammation and osteoclasts in mouse calvariae after exposure to wear particles.

Figure 2

A) The figures show representative microCT images of calvaria of mice treated with UHMWPE wear particles combined with anti-IgG (Control), anti-Netrin-1, anti-Unc5b or anti-DCC blocking antibodies at 10μg (n=5 mice per group). B) Calvariae were stained with hematoxylin & eosin to determine the presence of inflammation on the outer bone surface. The area of inflammatory infiltrate was quantified and expressed as a percentage of the area of the control particle-exposed mice (n = 5 mice per group). Shown are representative images for TRAP staining for osteoclasts in mice calvariae and the mean (±SEM, n=5 mice per group) number of osteoclasts/hpf. Images were taken at 400X magnification. Scale bar indicates 50 μm. Data were expressed as mean±SEM (n=5 per group). * p<0.05, **p<0.001, ***p<0.001 compared to control (ANOVA).