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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Ann Rheum Dis. 2015 Oct 9;75(9):1706–1713. doi: 10.1136/annrheumdis-2015-207593

Figure 4. Immunohistochemistry for markers of osteoblasts.

Figure 4

Calvariae were processed and immunohistologic staining carried out. Shown are representative representative H&E sections of calvaria (from n=5 mice per group) stained for Alkaline Phosphatase (green). Nuclei are shown in blue (Dapi). Quantification of the number of positive cellst/hpf was done. Data are means±SEM (n=5 mice per group). All images were taken at the same magnification 200X and 400X. Scale bar indicates 50 μm. ***p<0.001 (ANOVA).