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. 2017 Mar 14;8:275. doi: 10.3389/fimmu.2017.00275

Figure 1.

Figure 1

Monomeric IgA (mIgA) inhibits differentiation of human Th17 cells and affects the generation of IFN-γ+CD4+ T cells under Th17 differentiation conditions without modulating FoxP3+ T cells. (A) Flow cytometry analysis of intracellular IL-17A in the naïve CD4+ T cells cultured in serum-free X-vivo medium in the presence of anti-CD3 and anti-CD28 mAbs alone (medium) or stimulated with TGFβ and IL-21 for 6 days. mIgA (25 mg/ml), IVIG (25 mg/ml), or human serum albumin (HSA) (10 mg/ml) (0.15mM) were added to the T cell cultures after 12 h of cytokine stimulation. Data from one of five independent experiments are presented. (B) Percentage of IL-17A+CD4+ T cells (mean ± SEM, n = 5 donors) and (C) amount of secreted IL-17A (mean ± SEM, n = 9 donors) in T cell cultures differentiated under above conditions. mIgA was added at three different concentrations (5, 15, and 25 mg/ml). (D) Flow cytometry analysis of intracellular IFN-γ in the naïve CD4+ T cells under Th17 differentiation conditions. Data from one of five independent experiments are presented. (E) Percentage of IFN-γ+CD4+ T cells and (F) CD4+CD25+FoxP3+ T cells (mean ± SEM, n = 5 donors) among CD4+ T cells cultured under above conditions. Statistical significance as determined by one-way ANOVA is indicated (*P < 0.05; ***P < 0.001; ns, not significant).