Figure 2.

Isocratic elution of a size-exclusion chromatography (SEC) column to separate recombinant FcpA from L. biflexa as obtained after Ni²⁺-affinity purification. Elution volumes of standard globular proteins are overlaid with their molecular weights indicated to estimate the apparent molecular mass. Inset, 12% polyacrylamide electrophoresis gel run under denaturing conditions (SDS–PAGE) stained with Coomassie Blue. Molecular-weight markers (MWM) were separated in the leftmost lane; three of them are labelled (in kDa). A soluble protein extract from non-induced E. coli Rosetta-gami 2 (DE3) cells transformed with the pQE80_LbifcpA expression plasmid was separated in lane 1. Lane 2 is similar but after IPTG induction. Lane 3 shows the primary SEC peak.