| Method |
Hanging-drop vapour diffusion |
Hanging-drop vapour diffusion |
Sitting-drop vapour diffusion |
| Plate type |
VDX 24-well |
VDX 24-well |
96-well CrystalQuick |
| Temperature (K) |
293 |
293 |
293 |
| Protein concentration (mg ml−1) |
20 |
20 |
20 |
| Buffer composition of protein solution |
20 mM Tris–HCl pH 8.0, 300 mM NaCl |
20 mM Tris–HCl pH 8.0, 300 mM NaCl |
20 mM HEPES pH 7.0, 200 mM NaCl |
| Composition of reservoir solution |
20–25%(v/v) PEG 400, 100 mM Tris–HCl pH 7.5–8.5 |
3–5%(w/v) PEG 4000, 100 mM Tris–HCl pH 7.5–8.0 |
12%(w/v) PEG 8000, 0.1 M Na/K phosphate pH 6.2, 0.2 M NaCl |
| Volume and ratio of drop |
4 µl (1:1) |
4 µl (1:1) |
0.6 µl (1:1) |
| Volume of reservoir (ml) |
1 |
1 |
0.1 |
