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. 2017 Feb 21;73(Pt 3):130–137. doi: 10.1107/S2053230X17001911

Table 2. Crystallization.

Method Sitting-drop vapour diffusion
Plate type Intelli-Plate 96-2 shallow well (Hampton Research)
Temperature (K) 293
Protein concentration (mg ml−1) 19
Buffer composition of protein solution 10 mM Tris–HCl pH 8.0, 150 mM NaCl
Composition of reservoir solution 2 M ammonium sulfate, 0.1 M sodium citrate tribasic dihydrate pH 5.6, 0.2 M potassium sodium tartrate tetrahydrate
Volume and ratio of drop 300 nl protein solution, 300 nl reservoir solution
Volume of reservoir (µl) 50