Table 2. Crystallization.
Method | Sitting-drop vapour diffusion |
Plate type | Intelli-Plate 96-2 shallow well (Hampton Research) |
Temperature (K) | 293 |
Protein concentration (mg ml−1) | 19 |
Buffer composition of protein solution | 10 mM Tris–HCl pH 8.0, 150 mM NaCl |
Composition of reservoir solution | 2 M ammonium sulfate, 0.1 M sodium citrate tribasic dihydrate pH 5.6, 0.2 M potassium sodium tartrate tetrahydrate |
Volume and ratio of drop | 300 nl protein solution, 300 nl reservoir solution |
Volume of reservoir (µl) | 50 |