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. 2017 Feb 21;73(Pt 3):146–151. doi: 10.1107/S2053230X17002485

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Plate type 24-well
Temperature (K) 293
Protein concentration (mg ml−1) 12
Buffer composition of protein solution 20 mM Tris–HCl pH 8.0, 0.2 M NaCl
Composition of reservoir solution 0.1 M bis-tris pH 5.8, 20% PEG 3350, 0.2 M ammonium sulfate
Volume and ratio of drop 2 µl, 1:1
Volume of reservoir (µl) 700