| Method |
Hanging-drop vapour diffusion |
| Plate type |
24-well VDX plates (Hampton Research) |
| Temperature (K) |
Forms I and II, 293; form II′, 313 |
| Protein concentration (mg ml−1) |
10 |
| Buffer composition of protein solution |
20 mM potassium phosphate buffer pH 8.0 containing 0.01 mM PLP |
| Composition of reservoir solution |
Form I, 100 mM acetate pH 3.6, 54%(w/v) 2-methyl-2,4-pentanediol, 200 mM magnesium chloride, 10 mM ammonium sulfate; forms II and II′, 100 mM Tris–HCl pH 7.0, 12–18%(w/v) polyethylene glycol 3350, 200 mM sodium citrate, nickel(II) chloride hexahydrate |
| Volume and ratio of drop |
1.0 µl total, 1:1 ratio |
| Volume of reservoir (µl) |
400 |
