Table 2. Crystallization condition.

Method	Hanging-drop vapour diffusion
Plate type	0.5 ml sample cup (14 mm diameter × 24.6 mm) and siliconized cover glass
Temperature (K)	293
Protein concentration	10 mg ml−1 AfPAz and 5 mg ml−1 AfNIR (estimated from absorption at 280 nm)
Buffer composition of protein solution	20 mM Tris–HCl pH 8.0
Composition of reservoir solution	0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate trihydrate pH 6.5, 32.5–37.5%(w/v) PEG 8000
Volume and ratio of drop	1.5 µl:1.5 µl (protein solution:reservoir)
Volume of reservoir (µl)	400