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. 2017 Feb 28;73(Pt 3):167–173. doi: 10.1107/S2053230X17001376

Table 1. Cloning of expression constructs.

Source organism H. pylori
DNA source cDNA isolated from H. pylori 26695
Forward primer (FCagXct-EcoRI) CCGGAATTCCCCGTGCCTAGAAACTACAACTAC
Reverse primer (NCagXct-XhoI) CCGCTCGAGTTATGTCAATGGATTTTTCCCATAGCC
Cloning vector pET-32a
Expression vector pET-32a
Expression host E. coli BL21 (DE3)
Complete amino-acid sequence of the construct product PVPRNYNYYQAPEKRSKHIMPSEIFDDGTFTYFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIKDKALVTVINKGYGKNPLT