Table 1. Cloning of expression constructs.
Source organism | H. pylori |
DNA source | cDNA isolated from H. pylori 26695 |
Forward primer (FCagXct-EcoRI) | CCGGAATTCCCCGTGCCTAGAAACTACAACTAC |
Reverse primer (NCagXct-XhoI) | CCGCTCGAGTTATGTCAATGGATTTTTCCCATAGCC |
Cloning vector | pET-32a |
Expression vector | pET-32a |
Expression host | E. coli BL21 (DE3) |
Complete amino-acid sequence of the construct product | PVPRNYNYYQAPEKRSKHIMPSEIFDDGTFTYFGFKNITLQPAIFVVQPDGKLSMTDAAIDPNMTNSGLRWYRVNEIAEKFKLIKDKALVTVINKGYGKNPLT |