Fig. 5.

Determination of intracellular ROS and thiol content of S1 and S1-OE parasites exposed to ROS inducers (H₂O₂ and menadione). (A and B) Wild type (S1) & (S1-OE) parasites were exposed to lethal doses of H₂O₂ (25 µM, 50 µM and 100 µM) and menadione (1 µM, 2.5 µM and 5 µM) for 6 h and intracellular ROS were measured using Spectrofluorimeter. S1-OE parasites accumulated lesser intracellular ROS than wild type (S1) parasites upon exposure to lethal doses of oxidants. (C and D) Measurement of reduced intracellular thiol levels in wild type (S1) and S1-OE parasites after treatment with H₂O₂ and menadione for a period of 6 h. Results are expressed as means±SEM of three independent experiments. *, p<0.05; **, p<0.01; by Student's t-test using GraphPad Prism 5.0.