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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Nat Struct Mol Biol. 2016 Sep 26;23(11):995–1002. doi: 10.1038/nsmb.3296

Figure 2.

Figure 2

UFD-2 forms foci late after IR treatment. (a) Representative images of worm germlines of indicated genotypes irradiated with 60 Gy IR and stained with α-UFD-2 antibody and DAPI 24 hrs later. Filled arrowhead indicated nucleus with UFD-2 foci. Scale bar, 5 µm and (b) corresponding quantification of UFD-2 foci in pachytene region of germlines. Data show means ± s.e.m. of 12 independent experiments. (c) Representative images of worm germlines of indicated genotypes irradiated with 60 Gy IR. Germlines were isolated and stained with GFP-booster and DAPI 24 hrs after IR. Filled arrowheads indicate nuclei with UFD-2 foci. Scale bar, 5 µm and (d) corresponding quantification of UFD-2 foci in pachytene region of germlines. Data show means ± s.e.m. of 3 independent experiments. For n-values see Supplementary Table 1.

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