Table 2. Diversity metrics for SSU rRNA gene sequences obtained by 454 pyrotag sequencing.

Sample Site	Seal Pool			Atlas Pool			Brown Lagoon			Winston Lagoon			Atlas Cove
Location	−53.0183417°, 073.3922250°			−53.0195361°, 073.3895833°			−53.0690111°, 073.6648083°			−53.1503972°, 073.6760667°			−53.0125000°, 073.3706667°
Sample ID	509	510	511	490	489	488	572	571	570	543	542	541	538	539	540
Filter size (μm)	0.1	0.8	3	0.1	0.8	3	0.1	0.8	3	0.1	0.8	3	0.1	0.8	3
No. of sequences	36132	5323	5118	13121	26968	18150	12472	20162	7678	8118	8647	6832	9518	11990	8480
No. of OTUs at 97% similarity	859	287	271	337	650	416	87	277	99	392	218	388	176	317	365
Observed species* (Av. ± std. dev)	391 ± 10	283 ± 1	271 ± 0	237 ± 10	367 ± 9	275 ± 5	63 ± 5	151 ± 8	90 ± 3	339 ± 4	173 ± 6	363 ± 5	148 ± 3	244 ± 5	321 ± 6
Chao1* (Confidence interval)	719 (615–872)	337 (314–377)	289 (280–309)	360 (312–438)	579 (507–689)	406 (355–489)	93 (74–145)	281 (221–390)	115 (100–154)	441 (404–498)	263 (223–335)	431 (405–474)	198 (173–248)	360 (313–441)	396 (365–447)
Faith’s Phylogenetic diversity*	35.5	25.1	22.2	25.1	27.2	20.5	8.9	15.7	9.8	32.8	17.2	28.1	12.5	20.5	21.6
Shannon H’ *	4.46	5.46	5.61	4.10	5.75	5.13	2.51	2.79	3.50	4.52	3.38	5.34	4.11	5.10	6.49
Effective species (e^H’)*	87	235	272	60	314	168	12	16	33	92	29	210	61	164	660
Simpson (1-D)*	0.829	0.943	0.944	0.867	0.954	0.935	0.748	0.693	0.862	0.855	0.787	0.890	0.868	0.926	0.979

^*Alpha diversity calculations are based on rarefaction to 5118 sequences.