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. 2017 Mar 14;7:44260. doi: 10.1038/srep44260

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Copyright © 2017, The Author(s)

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(a) Immunofluorescence micrographs of IS and control osteoblasts at 0, 24, 48, and 72 hours following serum-starvation. Cells were stained for acetylated α-Tubulin (green), F-Actin (red), and Hoescht (blue). Long cilia (green) are visible in IS patients, at all time-points. (b) Elongated primary cilia appear more frequently in IS bone cells (4 IS vs. 4 controls assayed in duplicate, from 5 × 5 stitched tile images (50 fields) per sample). (c) Percentage of ciliated cells is not significantly different between IS and control cells (n ≤ 1,000 count per individual). Error bars are constructed using 1 standard error from the mean. Statistical analysis was performed with t-test using JMP-11, *P < 0.005.