Table 3. The basal level of gene expression in LiCl-treated control and IS compared to non-treated control osteoblasts.
| RQ (Fold change) | |||
|---|---|---|---|
| Control | LiCl-Control | IS | |
| BMP2 | 1 | 1.045 | 0.754 |
| PTGS2 | 1 | 3.942* | 1.481 |
| RUNX2 | 1 | 0.202* | 0.299* |
| SPP1 | 1 | 0.366* | 2.020* |
| CTNNB1 | 1 | 3.367* | 5.762* |
| GSK3B | 1 | 0.296* | 1.007 |
| INVS | 1 | 0.746 | 1.127 |
| AXIN2 | 1 | 0.821 | 0.508 |
| PTCH1 | 1 | 0.721 | 0.528 |
| GLI1 | 1 | Not Determined | 0.687 |
| ITGB1 | 1 | 1.670 | 1.169 |
| ITGB3 | 1 | 0.692 | 0.829 |
| POC5 | 1 | 0.499 | 1.133 |
The expression of several signaling and osteogenic factors were compared in human primary osteoblast samples from controls, LiCl treated controls and IS groups. The control cells are considered as the calibrator and the changes in gene expression of the other two groups are shown as the fold changes in relative quantity (RQ). LiCl-Controls were treated with 10 mM LiCl for 24 h. 2-fold change in gene expression compared to controls is considered significant and marked with an asterisks.