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The genomic regions of the island are highlighted, and the different fragments used for EMSAs are shown. Black fragments were bound by IolR, grey fragments not. The molar excess of protein over DNA is indicated above the gels. The argS (100 ng) promoter fragment of served as a negative control and competitor (c) DNA, and the first lane in each EMSA was loaded without IolR. Asterisks indicate fragments selected for SPR spectroscopy.
