| 14028 |
S. Typhimurium strain ATCC14028 |
ATCC |
| 14028 iolR::KanR
|
Allelic-exchange mutant |
This study |
| 14028 ∆iolR
|
In-frame iolR (STM4417) deletion mutant |
This study |
| 14028 dacB::KanR
|
Allelic-exchange mutant |
This study |
| 14028 iolR::KanR ∆4418–4436 |
Deletion of iolT1 (STM4418) to iolH (STM4436) in 14028 iolR::KanR
|
This study |
| MvP101 |
14028 with sseD::aphT, KanR; allelic-exchange mutant |
37 |
| MvP101 ∆iolR
|
In-frame iolR (STM4417) deletion mutant of MvP101 |
This study |
|
E. coli BL21(DE3) |
F−, ompT, hsdSB (rB−mB−), gal, lon, dcm, rne131, λ (DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5] |
38 |
| Plasmids |
| pKD3 |
pir-dependent, FRT sites, CmR
|
29 |
| pKD4 |
pir-dependent, FRT sites, KanR
|
29 |
| pKD46 |
Lambda-Red helper plasmid; AmpR
|
29 |
| pCP20 |
FLP recombinase plasmid; AmpR
|
29 |
| pUTs-gfp(CmR) |
Replacement of lux with gfp from pPROBE-NT in a transposase-negatvive derivate of pUT mini-Tn5 luxCDABE Km2; suicide plasmid, mobRP4, ori R6K, gfp, CmR
|
39 |
| pUTs-PiolR::gfp
|
pUTs-gfp(CmR) with the 500 bp region upstream of iolR (transcriptional fusion to gfp) |
This study |
| pUTs-iolR::gfp
|
pUTs-gfp(CmR) with the last 500 bp of iolR (translational fusion to gfp) |
This study |
| pUTs-PrpsM::gfp
|
pUTs-gfp(CmR) with the 500 bp region upstream of rpsM (STM3418) in front of gfp
|
This study |
| pBR-iolR
|
iolR with promoter region cloned into pBR322 |
2 |
| pET28b-iolR
|
Expression vector, T7lac promoter; KanR
|
2 |
