Fig 8. Deletion of Ift88 in early stages of forebrain development leads to increased brain size.

(A-L) Foxg1-Cre was used to delete a conditional allele of Ift88 and the forebrain was enlarged at E14.5 (B) and E18.5 (H,J,). Cortical architecture is disrupted at all stages examined (D,L) and fewer differentiated neurons are seen at E14.5 (F). (M-R) Similar, but less severe, phenotypes are seen with Emx1-Cre ablation at E14.5. (S-X) E18.5 mutants appear phenotypically normal. All paired images are at the same magnification. (Y) Quantification for brain sizes. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots; data points are plotted as open circles. n = 12, 6, 7, 5, 7, 4, 13, 4, respectively. (*:p = 0.011, **:p<0.005).