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A decreased level of ROS reversed the CHE-induced cell death, apoptosis, and autophagy in A549 and NCI-H1299 cells. (A–C) A549 and NCI-H1299 cells were treated with indicated concentration of CHE for 24 h with or without pretreatment with NAC (5 mM, 1 h). The dead cells were evaluated through annexin V-FITC/PI dual staining assay. The protein expressions of PARP, LC3-II, and GAPDH were evaluated by using Western blot assay. *P<0.05 and **P<0.01.
