Fig 3. SmPoMuc variant numbers in the different S. mansoni strains.

Nested RT-PCR for SmPoMucs was performed on 11 individual sporocysts for each S. mansoni strain (SmLE, SmVEN, SmBRE, and SmGH2), and the obtained fragments were cloned and analyzed by Sanger sequencing (see S1 Table). The numbers of SmPoMuc cDNA variants obtained for group 1 (red), group 2 (blue), group 3.1 (purple), group 3.1(r1-r2) (yellow), and all groups (black) are indicated.