FIGURE 4:

Proteotoxic phenotypes of cho2Δlpl1Δ and psd1Δlpl1Δ mutants. (A) Major classes of transcriptional targets for Hac1. (B) Growth of wild-type, lpl1Δ, cho2Δ, cho2Δlpl1Δ, dga1Δ, and dga1Δlpl1Δ strains in the presence of cadmium chloride (30 µM) or canavanine (1.5 µg/ml), as indicated. Cells were spotted in threefold serial dilutions and cultured at 30°C for 2–9 d. (C) Growth of wild-type, lpl1Δ, psd1Δ, and psd1Δlpl1Δ strains in the presence or absence of canavanine (1.5 µg/ml), as indicated. Cells were spotted in threefold serial dilutions and cultured at 30°C for 2–4 d.