FIGURE 1:

Adenoviral Cre efficiently ablated β_cyto- and γ_cyto-actin in primary MEFs. (A, B) Representative images of β_cyto-actin CT and β_cyto-actin KO cells at 7 dpi. Scale bar, 20 µm. (C–F) Representative qRT-PCR analysis of β_cyto- and γ_cyto-actin transcript amount (picomoles) in Actb^L/L, Actg1^L/L, and Actb^L/L Actg1^L/L MEFs. (G–I) Representative relative Western blot analysis of MEF lysates probed with β_cyto-actin and γ_cyto-actin antibodies; GAPDH served as loading control.