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. 2017 Mar 15;28(6):771–782. doi: 10.1091/mbc.E16-07-0503

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© 2017 Patrinostro et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — α_sm-Actin protein was up-regulated in siRNA-mediated β_cyto-actin knockdown MEFs. (A) Representative Western blot of CT and β_cyto- and/or γ_cyto-actin KD MEF lysates at 3 dpi for single KD and 4 dpi for dKD blotted with α_sm-actin, β_cyto-actin, and γ_cyto-actin antibodies; GAPDH served as loading control. (B–D) Quantitative Western blot analysis of control and KD MEF lysates (n = 3). Calculated protein concentrations (nanograms/microliter lysate) were determined based on the standard curve, blotted in parallel. ***p < 0.001. Two-way ANOVA with Bonferroni posttest; error bars are SEM.