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. 2017 Mar 15;28(6):771–782. doi: 10.1091/mbc.E16-07-0503

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© 2017 Patrinostro et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 9: — Caldesmon smooth muscle isoform protein expression was up-regulated in β_cyto-actin–deficient MEFs. (A) Representative Western blot analysis of CT and KO MEFs blotted with caldesmon (Cald1_sm), CNN1, Sm22α, MLC2, and pMLC2; GAPDH served as loading control. (B–D) Relative protein expression normalized to GAPDH and relative to the paired embryo control. *p < 0.05, ***p < 0.001. One-sample t test, error bars are SEM.