FIGURE 2:

CRY deficiency affects IGF-1 level in plasma and tissues. (A) Daily rhythms of plasma IGF-1 in WT AL (blue diamonds) and Cry1, 2−/− AL (red squares) males (N = 3 per time point). (B) Plasma IGF-1 levels for WT (blue bars), Cry1−/− (red bars), Cry2−/− (green bars), and Cry1, 2−/− (purple bars) males at ZT6 and ZT18 (N = 6 per time point). (C) Representative Western blotting (pooled extracts from three mice per time point) of IGF-1 expression in the liver (left) and skeletal muscle (right) of WT and Cry1, 2−/− male mice. (D) Quantification of IGF-1 in the liver (left) and skeletal muscle (right) of WT AL (blue diamonds) and Cry1, 2−/− (red squares) male mice (N = 3 per time point). The quantification data for the liver and skeletal muscles are presented as relative arbitrary units; note that these units are different for the liver and skeletal muscle graphs because the levels of IGF1 protein and Igf1 mRNA in the liver are orders of magnitude higher than in skeletal muscle. Different exposure time was used for Western blots in the liver and skeletal muscles. *Statistically significant difference between genotypes (p < 0.05). Light was on at ZT0 and off at ZT12.