TABLE 1.

Structural elucidation and biosynthesis of novel bioactive mediators

Chemical and biochemical procedures used to deduce structures of bioactive SPMs
Precursor analysis, 14C label incorporation
MS-MS spectra and after deuterium incorporation
GC-MS of several derivatives, trapping intermediates
MS spectra of several derivatives
UV chromophore
Confirmation total organic synthesis, physical properties, and biologic function

Bioactions and function of proresolving mediators
Limit PMN tissue infiltration, cessation
Reduce collateral tissue damage by phagocytes
Shorten Ri resolution interval
Enhance macrophage phagocytosis, and efferocytosis
Counterregulate proinflammatory chemical mediators (PAF, LTs, PGs)
Increase anti-inflammatory mediators (IL-10 and others)
Increase microbial killing and clearance by innate immune cells
Enhance tissue regeneration