Figure 5.
The production of 5-LOX-related lipid AA derivatives from lysates of 5-LOX-transfected HEK cells exposed to propofol at different concentrations. A) Lysates of 5-LOX transfected HEK cells were stimulated with AA and Ca2+ in the presence of propofol at various concentrations, and the supernatant was subjected to lipid mediator analysis. Cluster analysis of lipid mediators was performed with MEV software, with the results shown on a heat map. Lipid mediators clustered with LTB4 are highlighted. B) IC50 of propofol was determined. Data are means ± sd of 4 replicates.