TABLE 1.
DNA templates and primers for cloning
| Construct | DNA template | Primers | Vector |
|---|---|---|---|
| pcDNA3-AXL-Flag | AXL cDNA | 1, 2 | pcDNA3 |
| pCDH-AXL-Flag | AXL cDNA | 3, 4 | pCDH-CMV |
| pcDNA3-MERTK-Flag | MERTK cDNA | 5, 6 | pcDNA3 |
| pcDNA3-TYRO3-Flag | TYRO3 cDNA | 7, 8 | pcDNA3 |
| pET21b-AXL(428-527)-Flag | AXL cDNA | 9, 11 | pET21b |
| pET21b-AXL(445-527)-Flag | AXL cDNA | 10, 11 | pET21b |
| pCDH-AXL(473-894)-Flag | AXL cDNA | 12, 4 | pCDH-CMV |
| Δ1 (AXL428-435) | pCDH-AXL-Flag | 13, 14 | pCDH-CMV |
| Δ2 (AXL436-443) | pCDH-AXL-Flag | 15, 16 | pCDH-CMV |
| Δ3 (AXL444-451) | pCDH-AXL-Flag | 17, 18 | pCDH-CMV |
| L437A | pcDNA3-AXL-Flag | 19, 20 | pcDNA3 |
| V438A | pcDNA3-AXL-Flag | 21, 22 | pcDNA3 |
| L437A/V438A | pcDNA3-AXL-Flag | 23, 24 | pcDNA3 |
| mut1 | pCDH-AXL-Flag | 25, 26 | pCDH-CMV |
| mut2 | pCDH-AXL-Flag | 27, 28 | pCDH-CMV |
| mut3 | pCDH-AXL-Flag | 29, 30 | pCDH-CMV |
| mut4 | pCDH-AXL-Flag | 31, 32 | pCDH-CMV |
| mut1a | pCDH-AXL-Flag | 33, 34 | pCDH-CMV |
| mut1b | pCDH-AXL-Flag | 35, 36 | pCDH-CMV |
| mut1c | pCDH-AXL-Flag | 37, 38 | pCDH-CMV |
| V459A | pcDNA3-AXL-Flag | 39, 40 | pcDNA3 |
| V472A | pcDNA3-AXL-Flag | 41, 42 | pcDNA3 |
| V459Δ | pcDNA3-AXL-Flag | 43, 44 | pcDNA3 |
| V472Δ | pcDNA3-AXL-Flag | 45, 46 | pcDNA3 |
| V458L | pcDNA3-AXL-Flag | 47, 48 | pcDNA3 |
| A468T | pcDNA3-AXL-Flag | 49, 50 | pcDNA3 |
| V458L/A468T | V458L | 49, 50 | pcDNA3 |
| K567R | pcDNA3-AXL-Flag | 51, 52 | pcDNA3 |
| Flag-AXL | pCDH-AXL-Flag | 53, 54 | pCDH-CMV |
| AXL(473-894)-EGFP | AXL cDNA | 55, 58 | pEGFP-N2 |
| AXL(481-894)-EGFP | AXL cDNA | 56, 58 | pEGFP-N2 |
| AXL(531-894)-EGFP | AXL cDNA | 57, 58 | pEGFP-N2 |
| AAAAA | AXL(473-894)-EGFP | 59, 60 | pEGFP-N2 |
| AAAAK | AXL(473-894)-EGFP | 61, 62 | pEGFP-N2 |
| HAAAA | AXL(473-894)-EGFP | 63, 64 | pEGFP-N2 |
| AAKK | AXL(473-894)-EGFP | 65, 66 | pEGFP-N2 |
| RAAK | AXL(473-894)-EGFP | 67, 68 | pEGFP-N2 |
| RRAA | AXL(473-894)-EGFP | 69, 70 | pEGFP-N2 |
| RRKA | AXL(473-894)-EGFP | 71, 72 | pEGFP-N2 |
| RRAK | AXL(473-894)-EGFP | 73, 74 | pEGFP-N2 |
| RAKK | AXL(473-894)-EGFP | 75, 76 | pEGFP-N2 |
| ARKK | AXL(473-894)-EGFP | 77, 78 | pEGFP-N2 |
| NF-κB-Luc | 5× κB response element | 79, 80 | pGL3 Basic |
Primer values correspond to no. in Supplemental Table 1.