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Oxygen- and GOT-sensitive changes in anaplerosis. A) Experimental protocol. B) [U-13C] propionate enters the TCA cycle at the level of succinyl CoA. C) Representative GC-MS spectra of control (cont) under RA. D) Quantification of anaplerosis under all conditions. Data are mean ± sd fold change compared with RA (n/group: RA = 4, RA pLV con = 3, RA pLV GOT = 7, SO = 8, SO pLV con = 4, SO pLV GOT = 4). *P < 0.05 vs. RA.
