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. 2015 Oct 26;7(52):85750–85763. doi: 10.18632/oncotarget.5509

Figure 4. Tubulogenesis of human U87 glioblastoma cells is inhibited by GMF-β knockdown.

Figure 4

A. Protein levels of GMF-β in human glial cell line (HEB) and human glioma cell lines of the different grades (CHG5, SHG44, U87). B. Assessment of GMF-β knockdown in U87 cells by western blotting. C. Tube formation by U87 mock cells (left panel); impaired tube formation by U87-shGMF-β cells (right panel). Inspection under a phase contrast fluorescent microscope (× 100). D. Quantified tubulogenesis of U87 mock cells and U87-shGMF-β cells by pattern recognition system (left panel) and branch point counting system (right panel).*** indicates significant difference with P < 0.001.