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. 2016 Nov 3;7(52):85848–85860. doi: 10.18632/oncotarget.13039

Figure 1. Amount of ATP in cell lysates and NAD(P)H production in cells from the line U87 under different culture conditions.

Figure 1

Cells from the line U87 were cultivated for 20 hours at a density of 5000 cells per well in 96-well microplates in DMEM standard culture medium before they received fresh medium with different supplements: FBS: fetal bovine serum, 10%; Glx: GlutaMax, 2 mM; Glu/Gal/Pyr: glucose, galactose, pyruvate: each 25 mM; 0: no Glu/Gal/Pyr. 24 hours later the CellTiter-Glo assay (measuring ATP in cell lysates, left part of the panel) and the CellTiter-Blue assay (measuring NAD(P)H production, right part of the panel) were employed to assess cell viability. Signals obtained from cells cultivated in 25 mM glucose, 10% FBS and 2 mM GlutaMax were set as 100%. Results are presented as mean and standard deviation of 6 wells. Statistical significance was determined by Student's t-test with: *p < 0.05; **p < 0.005; ***p < 0.0005.