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. 2016 Nov 3;7(52):85848–85860. doi: 10.18632/oncotarget.13039

Figure 4. Amount of ATP in cell lysates of the lines U87, T98G and LN405 under the influence of different concentrations of carnosine in the presence of glucose and glucose plus pyruvate.

Figure 4

Cells were seeded at a density of 5000 cells per well in 96-well microplates and received medium without glucose, galactose or pyruvate and without FBS and GlutaMax. After 20 hours, fresh medium was added with either glucose (Glu; 25 mM) or a mixture of 25 mM glucose and 5 mM pyruvate (Glu+Pyr) and cell line dependent inhibitory concentrations of carnosine (see Supplementary Data S2). 24 hours later the amount of ATP in cell lysates was determined using the CellTiter-Glo assay. The amount of ATP in the absence of carnosine was set as 100%. Results are represented as mean and standard deviation of 6 wells for each condition. Statistical significance was determined by Student's t-test with: *p < 0.05; **p < 0.005; ***p < 0.0005.