Skip to main content
. 2016 Nov 11;7(52):85948–85962. doi: 10.18632/oncotarget.13288

Figure 4. MAPK inhibition in thyroid cancer cells disrupts the binding of FOXE1 to ELK1.

Figure 4

(A) Determination of the effect of MEK inhibition upon the FOXE1-ELK1 interaction. SW1736 cells were transiently transfected with various combinations of FOXE1-Flag and WT/mutant ELK-HA, treated with 10 μM MEK inhibitor or vehicle control, and then immunoprecipitation was performed using an anti-Flag antibody, and the western blot probed with an anti-HA antibody. (B) Mammalian two-hybrid assay in SW1736 cells using transfected Gal4-FOXE1 and ELK1-VP16 and pGL5-luc reporter. Twenty-four hours post-transfection, the cells were treated with 10 μM U0126 or vehicle control for a further 24 hrs, prior to the harvest of protein lysates and subsequent reporter assay. Values are the the mean (± SD) of three experiments, each performed in triplicate, expressed as fold increase in luciferase activity relative to cells transfected only with reporter. Significant changes are highlighted (*p < 0.05, Student’s t-test).