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. 2016 Dec 11;7(52):86039–86050. doi: 10.18632/oncotarget.13325

Figure 2. IFITM1 depletion modestly impairs cell proliferation in colorectal cancer cell lines.

Figure 2

Colorectal cancer cell lines were infected with either control (shLacZ) virus or IFITM1 knockdown virus (642-shIFITM1 and 870-shIFITM1) and selected with puromycin. RNA and proteins were isolated to determine the expression of IFITM1. (A) RT-qPCR was performed to determine IFITM1 mRNA expression in SW620 cells after infection. (*p < 0.05). (B) Immunoblot with anti-IFITM1 antibody was conducted to analyze IFITM1 protein level, and ACTIN was used as the loading control. (C–E) Control (shLacZ) or IFITM1-depleted (shIFITM1) colorectal cancer cell lines were incubated for 72 hrs to determine the proliferation rate by MTT assay. Data shown are from two to three experiments (*p < 0.05, **p < 0.001).