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. 2016 Dec 11;7(52):86039–86050. doi: 10.18632/oncotarget.13325

Figure 3. IFITM1 depletion impairs migration and invasion of colorectal cancer cell lines in vitro.

Figure 3

Control (shLacZ) or IFITM1-depleted (shIFITM1) colorectal cancer cell lines were seeded in a matrigel-uncoated and coated transwell, followed by incubation for 18 hrs and 24 hrs for assessing migration and invasion, respectively. Cells that had migrated to the lower surface of the transwell were stained and quantified. (A and E) Imaging was done using an inverted microscope (magnification: 100×) and representative images are shown. Data shown (A–D: migration; E–H: invasion) are from three experiments (*p < 0.05, **p < 0.001).