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. 2016 Dec 11;7(52):86039–86050. doi: 10.18632/oncotarget.13325

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Copyright: © 2016 Sari et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A–D) RNA was isolated from control (shLacZ) or IFITM1-depleted (shIFITM1) cancer cell lines (A and B: HT29; C and D: SW620) and EMT signature genes were determined by RT-qPCR (*p < 0.05, **p < 0.001). (E) Cell lysates were obtained from control (shLacZ) or IFITM1-depleted (shIFITM1) HT29 cells and immunoblots were conducted with antibodies indicated. ACTIN was used as a loading control. (F) Supernatants obtained from control (shLacZ) or IFITM1-depleted (shIFITM1) HT29 cells were analyzed for enzymatic activity of MMP1/3, MMP2 and MMP9.