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. 2016 Dec 11;7(52):86039–86050. doi: 10.18632/oncotarget.13325

Figure 6. Ectopic expression of IFITM1 promotes the proliferation and migration of SW620 cancer cells in vitro.

Figure 6

(A and B) SW620 cells were transiently transfected with either control (vector) or IFITM1 expression construct (IFITM1 OE). RNA and proteins were isolated to examine the expression of IFITM1. (A) RT-qPCR was performed to determine IFITM1 mRNA expression in SW620 cells after transfection (**p < 0.001). (B) IFITM1 protein level was analyzed by immunoblot using anti-IFITM1 antibody, and ACTIN was used as the loading control. (C) SW620 cells transfected with control (vector) or IFITM1-expression construct (IFITM1 OE) were incubated for 72 hrs to determine the proliferation by MTT assay. Data shown are from three experiments (**p < 0.001). (D and E) SW620 cells transfected with control (vector) or IFITM1-expression construct (IFITM1 OE) were seeded in a transwell for assessing migration. Cells were incubated for 18 hrs, stained, and quantified. (D) Representative images are shown. (E) Data shown are from three experiments (*p < 0.05). (F and G) SW620 cells were transiently transfected with either Vector alone, IFITM1-overexpressing (IFITM1 OE) and Vector, or IFITM1 OE and CAV1-overexpressing (CAV1 OE) constructs. RNA was isolated from the transduced cells, and CAV1 (F) and EMT signature (G) were quantified by RT-qPCR (*p < 0.05, **p < 0.001).