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. 2016 Nov 14;7(52):86087–86102. doi: 10.18632/oncotarget.13343

Figure 5. RER treatment attenuated TGF-β signaling in vivo.

Figure 5

(A) Western blot analysis of p-Smad2, p-Smad3, T-Smad2/3, E-cadherin, Vimentin in anterior prostate (AP) from all the experimental mice as indicated. The bar plots are the total Smad2 or Smad3-normalized p-Smad2 or p-Smad3 levels and the GAPDH-normalized E-cadherin and Vimentin for each sample quantified with Protein Simple software. Each data bar represents the mean±SEM from six samples. (B) qRT-PCR analysis of mRNA expression of E-cadherin and Vimentin in dorsal and ventral prostate of all the experimental mice. (C, D, E) IHC staining of p-Smad2, Vimentin, and E-cadherin in liver and kidney tissue of the experimental mice. A representative picture was randomly taken for each staining from tissue sections of three mice in each group.