Figure 3. NDV entry into DF1 cells is dynamin-dependent, whereas plasma membrane cholesterol is dispensable.

(A–B) CLSM imaging revealed the inhibitory effect of MbCD on CTxB uptake (red; upper left panels), whereas NDV uptake was not effectively blocked (upper right panels). (C–D) Western blotting showing inhibition of NDV entry by MbCD or dynasore. The ratio of NP/b-actin is shown. (E) Dynasore inhibition of NDV entry observed using CLSM: actin filaments (red), NP protein (green), cell nucleus (blue). (F) NDV titers in the supernatant of Mock- or dynasore-treated cells were determined using the TCID₅₀ assays. The results are presented as the mean ± SD, *P < 0.05. Scale bar = 20 μm.