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. 2016 Nov 15;7(52):86134–86147. doi: 10.18632/oncotarget.13348

Figure 1. Expression of GLI1 in colorectal cancer tissues and cell lines.

Figure 1

(A) Immunohistochemical results of Gli1 expression in CRC tissues: Gli1 was positively stained in the cytoplasm of the majority of tumor specimens and was negatively or weakly stained in the adjacent normal tissues. (B) Gli1 expression levels were assessed in tumor tissues and adjacent normal tissues. The ΔCT value was determined by subtracting the GAPDH CT value from the Gli1 CT value. Smaller ΔCT value indicates higher expression. (C) Gli1 expression levels were measured by qRT-PCR and WB together with Foxm1 in CRC cells. The intensity of the bands was determined using densitometric analysis.**p < 0.01, *p < 0.05.