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. 2016 Nov 11;7(52):86211–86224. doi: 10.18632/oncotarget.13367

Figure 4. Effect of DS2 treatment for 24 h on EC109 cells, HEECs and normal human liver cells (HL-7702).

Figure 4

(A) and (B) After EC109 cell and HEECs were treated with 0.1% DMSO or DS2 at the indicated doses for 24 h, the cells were stained with FITC-Annexin V/PI and then analyzed by flow cytometry. (C) and (D) Percentages of cells with early apoptosis or late apoptosis were summarized with histogram graphs. The experiments were repeated three times and the results were presented as mean ± SD. *P < 0.05 and ** P < 0.01 as compared with control. (E) and (F) Survival rates of HEECs and HL-7702 cells are determined by trypan blue dye exclusion assay. Data are presented as means ± SD of triplicate samples. *P < 0.05;**P < 0.01 as compared with control.