Figure 1. Setbp1 missense mutants confer more efficient immortalization to bone marrow progenitors compared to wild-type Setbp1.

(A) Mean and SD of colony numbers formed by 5 × 10³ 5-fluorouracil (5-FU) treated bone marrow (BM) progenitors from C57BL/6 mice transduced with pMYs-IRES-GFP, pMYs-Setbp1-IRES-GFP, pMYs-Setbp1(I/T)-IRES-GFP, or pMYs-Setbp1(D/N)-IRES-GFP retrovirus. Infected cells were sorted by GFP expression and plated on methylcellulose medium in the presence of stem cell factor (SCF) (100 ng/ml), interleukin (IL)-6 (10 ng/ml), and IL-3 (6 ng/ml). Cells were re-plated every 5–7 days. (B) Wright-Giemsa staining of cells immortalized by transduction with retroviruses expressing the indicated wild-type or mutant Setbp1 after passaging in liquid media containing SCF and IL-3 for 2.5 months. *P < 0.05; **P < 0.01; ***P < 0.001 (two-tailed Student’s t test).