Figure 7. Dysregulated hTDP1 co-treated with topotecan enhances cytotoxicity.

Exponentially growing stably transfected HEK293 cells with indicated HAhTDP1 allele or control cells (vector) were seeded 24 hours prior to treatment schedule. A-B. Cells were treated with or without Dox 24 hours prior to topotecan (TPT) treatment of 0.1 to 100 nM. C-D. Cells were treated with TPT (0.1 to 100 nM) 24 hours prior to ± Dox treatment. 4 days after last treatment cell viability was determined using Allamar blue. Shown is the average and standard deviation of at least 3 independent experiments. A-C. no hTDP1 expression was induced; B-D. hTDP1 expression was induced by 0.1 μg /mL Dox.