Table 1. Samples tested for methylation of the Lgr6 promoter.
| sample group | # samples tested | # with part ofLgr6 promotersmethylated | % samples showingmethylation |
|---|---|---|---|
| Skin untreated (mice with wt Lgr6 locus) | 10 | 0 | 0 |
| skin untreated (Lgr6-EGFP-Ires-CreERT2/R26R-LacZ mice) | 16 | 7 | 44 |
| skin treated with UV 30 days 1 MED (mice with wt Lgr6 locus) | 4 | 0 | 0 |
| skin treated with UV 4-8 weeks 1 MED (Lgr6-EGFP-Ires-CreERT2/R26R-LacZ mice) | 14 | 6 | 43 |
| inter-tumoral skin (Lgr6-EGFP-Ires-CreERT2/R26R-LacZ mice) | 16 | 14 | 88 |
| Tumors (Lgr6-EGFP-Ires-CreERT2/R26R-LacZ mice) | 27 | 26 | 96 |
| Tumors (mice with wt Lgr6 locus) | 12 | 0 | 0 |
Aberrant methylation was detected by MS-MCA. We tested samples from mice with a wt Lgr6 locus and samples from haired and hairless Lgr6-EGFP-Ires-CreERT2/R26R-LacZ mice. Samples of these last two groups were lumped together since no differences were observed; the same was true for tumors from the chemical and UV carcinogenesis experiments. None of the samples from the Lgr6-EGFP-Ires-CreERT2/R26R-LacZ mice showed complete methylation of Lgr6 promoters, next to a peak from methylated promoters there was always a peak from unmethylated promoters.