Skip to main content
. 2016 Nov 24;7(52):86755–86765. doi: 10.18632/oncotarget.13550

Figure 4. Overexpression of TRIM11 promotes CC cell proliferation and inhibits cell apoptosis.

Figure 4

A. The generation of stable cell lines in which TRIM11 was overexpressed or silenced was confirmed by western blotting. GAPDH was used as the internal control. B, C. The cell proliferation of the indicated stable cell lines in vitro was measured at different time points, as indicated by the CCK-8 assay. The bars correspond to the mean± standard error, and the P value was calculated using Student's t-test. *P<0.05, **P<0.01. D, E. The colony formation of the indicated stable cell lines in vitro was measured for 14 days. The bars correspond to the mean± standard error, and the p-value was calculated using Student's t-test. **P<0.01,***P<0.001. F, G. The stable cell lines overexpressing the empty vector or TRIM11 were treated with 150 μg/ml5-FU for 24 h and then subjected to annexin V-FITC and PI staining. Cell apoptosis was evaluated through FACS. The bars correspond to the mean± standard error (n = 3), and the P-value was calculated using Student's t-test. *P<0.05.